camp elisa kit  (Cusabio)

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: The figures illustrate the docking interaction of paeoniflorin with the GDNF receptor (PDB ID: 1AGQ). (A) Shows a surface representation of the GDNF receptor, with the active site highlighted in yellow, where the ligand paeoniflorin (red) is bound. (B) 2D interaction diagram displays key interactions between paeoniflorin and the receptor, with hydrogen bonds and hydrophobic interactions clearly indicated. (C) Provides a 3D view of the docking, showing paeoniflorin (blue) interacting with GDNF, with hydrogen bonds represented by green dashed lines and key regions labeled. (D) Offers a zoomed-in view of the binding site, emphasizing hydrogen bonds and hydrophobic contacts, depicted within a ribbon structure for clarity.

Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

Techniques: Labeling, Binding Assay

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–H) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of cellular and molecular targets in MS rat model: GDNF (A) , GFRA1 (B) , AKT (C) , ERK1/2 (D) , GSK3-Beta (E) , in brain homogenates, and GDNF, GFRA1, AKT, ERK1/2, GSK3-Beta in CSF levels (F–H) . Statistical analysis was performed using a one-way ANOVA followed by Tukey’s post hoc test to determine significant differences among groups (A–H) . Data were presented as mean ± standard deviation (SD), with statistical significance set at p < 0.01. Each experimental group consisted of eight wistar rats (n = 8). β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–H) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

Techniques: Standard Deviation, Control

Journal: Journal of Advanced Research

Article Title: A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy

doi: 10.1016/j.jare.2025.05.063

Figure Lengend Snippet: The in vitro release behavior of neural inducers and biocompatibility of BGA@GelMA hydrogel. (A to C) The relative release profile of BDNF, GDNF, and cAMP of the BGA@GelMA hydrogel measured with ELISA kit, n = 3. (D) Schematic representation of 3D culture system via the BGA@GelMA and the Matrigel hydrogel. (E) Flow cytometry was used to detect APC of cell apoptosis. (F) Statistical diagram of apoptotic cell distribution, n = 3, *** p = 0.0005. (G) Quantitative analysis of live cells and dead cells per field in the live/dead assay, n = 3. (H) Representative live/dead images of hNPCs co-cultured with the BGA@GelMA hydrogels and the Matrigel hydrogels after 14 days.

Article Snippet: The release kinetics of neural inducers in BGA@GelMA hydrogel were assessed using the human BDNF valukine enzyme-linked immunosorbent assay (ELISA) kit (VAL136, R&D Systems), GDNF ELISA kit (CSB-E04565h, Cusabio) and cAMP ELISA kit (CSB-E04488h, Cusabio), respectively.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay, Cell Culture

Journal: Journal of Advanced Research

Article Title: A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy

doi: 10.1016/j.jare.2025.05.063

Figure Lengend Snippet: The in vitro release behavior of neural inducers and biocompatibility of BGA@GelMA hydrogel. (A to C) The relative release profile of BDNF, GDNF, and cAMP of the BGA@GelMA hydrogel measured with ELISA kit, n = 3. (D) Schematic representation of 3D culture system via the BGA@GelMA and the Matrigel hydrogel. (E) Flow cytometry was used to detect APC of cell apoptosis. (F) Statistical diagram of apoptotic cell distribution, n = 3, *** p = 0.0005. (G) Quantitative analysis of live cells and dead cells per field in the live/dead assay, n = 3. (H) Representative live/dead images of hNPCs co-cultured with the BGA@GelMA hydrogels and the Matrigel hydrogels after 14 days.

Article Snippet: The release kinetics of neural inducers in BGA@GelMA hydrogel were assessed using the human BDNF valukine enzyme-linked immunosorbent assay (ELISA) kit (VAL136, R&D Systems), GDNF ELISA kit (CSB-E04565h, Cusabio) and cAMP ELISA kit (CSB-E04488h, Cusabio), respectively.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay, Cell Culture